In this study, our goal was to use this mIF panel and apply it in a small cohort of a lung adenocarcinoma mouse model to explore the versatility of detecting co-expression markers, especially the axis of PD-1/PD-L1, TILs and macrophages to see the ability of this data to explore the spatial distribution of different cell subtypes. Here, PDCD1 is linked to lung adenocarcinoma.