Although G6PDS188F variant reduced the severity of NAFL, it did not rescue the expression of Bmal1. Instead, we found expression of Magel2, a gene encoding circadian clock-related protein that suppresses obesity and metabolic reprogramming associated with Prader-Willi syndrome (31), increased in HFD-fed G6PDS188F rats as compared with wild-type rats (Fig. 5E). This evidence concerns the gene BMAL1 and obesity due to melanocortin 4 receptor deficiency.