Since increased 2-hydroxyglutarate contributes to the development of oxidative stress inhibits chromatin-modifying enzymes and mTOR in cancer (53) and elicits inflammation (49), we predict 2-hydroxyglutatrate-associated oxidative stress and inhibition of epigenetic enzymes potentially contribute to the progression of HFD-induced NAFL. Here, MTOR is linked to non-alcoholic fatty liver.