Beyond the STN, the DRN is also a brain area known to be activated in migraine.88 The EWcp/UCN1 neurons innervate the DRN,23,97,109 where both CRH receptors are expressed.14,64,96,97 Activation of CRH1R in DRN reduces 5-HT release, whereas CRH2R signaling has opposite effect.33,63 The decreased DRN/5-HT content in our CGRP-treated mice suggests that 5-HT release upon CGRP administration. This evidence concerns the gene CRHR2 and migraine disorder.