Although migraine is more prevalent in females,83 we used male mice because (1) EWcp/UCN1 neurons express estrogen receptor beta and estrous cycle-related hormonal fluctuations modulate UCN1 levels18,19 and (2) 0.1 mg/kg CGRP induced migraine-like symptoms in both male and female mice.68,81 Our fMRI study recruited male and female migraineurs only during the interictal period; thus, the results do not reflect the brain status during the active headache episodes. Here, ESR2 is linked to migraine disorder.