HFE and hereditary disease: The iron storage disorder hereditary hemochromatosis (HHC) is an autosomal recessive genetic disorder commonly caused by a G to A mutation at nucleotide 845 in the human HFE gene resulting in a C282Y substitution in the HFE protein that leads to abnormal protein function with excessive iron absorption and potentially life‐threatening elevation of serum ferritin.[15, 27] There was only one sgRNA with canonical NGG PAM can be used containing the target adenine at the +5 position and two missense mutations at the +1 (ACG→GCG) or +8 (CAG→CGG) position of the protospacer in HHC genome (Figure4a).