To determine if there is a change in state of the macrophages that express TIE:EGFP (either endogenously or via phagocytosis), we subset macrophages from the SATB2 expressing melanoma and separated macrophages that were TIE:EGFP+ or TIE:EGFP- based on flow cytometry intensity. The gene discussed is SATB2; the disease is melanoma.