MITF and melanoma: To understand the transcriptional differences between TIE:EGFP+ and TIE:EGFP- melanoma cells, single TIE:EGFP+ and mitfa:mCherry+ cells from TIE:EGFP expressing melanomas were processed for single cell RNA-sequencing at 23 and 42 weeks post fertilization (wpf), respectively (melanomas used are depicted in Figure 1C and Figure 1—figure supplement 2B).