RT-qPCR analysis of ARF expression demonstrated a significant increase in ARF mRNA levels in SIRT7-depleted H1299 cells but not in Calu-3 cells (SI Appendix, Fig. S1H), indicating that SIRT7 not only influences ARF stability but also interferes with gene expression or mRNA stabilization at least in some lung cancer cells. The gene discussed is SIRT7; the disease is lung cancer.