In brief, CAR T cell manufacturing included depletion of CD14+ and CD25+ cells, CD3/28 bead stimulation, transduction with lentivirus at multiplicity of infection of 0.3, removal of beads at day 7–9, followed by expansion for a total of 12–17 days in interleukin (IL)-2 and IL-15 cytokines. This evidence concerns the gene IL2 and infection.