ST8SIA1 and neuroblastoma: We also analyzed the parental vs. GD3S-KO RM-1 and 22Rv1 cell lines together with GD2+ 9464D and GD2- 975A2 mouse neuroblastoma cell lines41 by thin layer chromatography (TLC) and TLC/immunoblotting, in which lipid extracts of cells were resolved by TLC next to the purified GD2 standard, followed by either chemical staining to visualize the separated sphingolipid species or immunoblotted with the 14G2a antibody, respectively.