TGFB1 and melanoma: We confirm that gene expression involved in pathways that are integral for melanoma growth and drug resistance, including MAPK and phosphatidylinositol signaling,20,32,33,34,35,36 and processes that we have previously identified in CSF fluid to be important for melanoma LMD, such as TGF-β signaling and complement and coagulation,5,37 are not distributed randomly throughout the tissue but are rather correlated to the proximity of the tumor to the stroma.