We adopted the CRISPR-assisted detection of RNA-protein interactions (CARPID) method,38 utilizing guide RNAs that were specific for both linear and circular NFIX in a colorectal adenocarcinoma cell line, DLD-1 cells, as outlined in Figure 5A. Three biological replicates were performed for each pull-down experiment. Here, NFIX is linked to colorectal adenocarcinoma.