To test the potential of MISs in MIC detection, mIHC staining was used to detect the co‐expression patterns of 4 selected MISs (CD44, S100A14, RHOD, and TACSTD2) in four ESCC cell lines (KYSE30, KYSE180, KYSE410, and KYSE520). The gene discussed is TACSTD2; the disease is esophageal squamous cell carcinoma.