Our study has depicted the specific MICs subpopulation in ESCC via multiplex staining of the simultaneous co‐expression of our MISs panel (i.e., CD44, S100A14, RHOD, and TACSTD2) in cell lines and illustrated the enrichment of MICs in metastatic mice lungs along timeline. This evidence concerns the gene TACSTD2 and esophageal squamous cell carcinoma.