Prior to the purification of exhaled EVs, we also compared the species specificity of our anti-human and anti-mouse anti-CD63 antibodies by Western blot analyses of EVs ultracentrifuged from tissue culture-derived media of human MDA-MB-231 breast cancer cell, human HEK293 cells, and primary mouse bone marrow-derived endothelial cells (BMEC), which revealed high species-specific recognition of our anti-human and anti-mouse anti-CD63 antibodies [Figure 7A]. This evidence concerns the gene CD63 and breast cancer.