To this aim, we established a pull-down assay in which we incubated a lysate of SH-SY5Y human neuroblastoma cells with a biotinylated sequence of the shortest tRF upregulated in Klotho KO, tDR-36:74-Asn-GTT-2-M2, representing the shared sequence between the three upregulated fragments. This evidence concerns the gene KL and neuroblastoma.