Notably, two DE miRs in Klotho KO showed cell type-dependent age correlation (Fig. 5e) The cholinergic miR-335-5p, which targets the muscarinic receptors and has been proposed to limit the cholinergic blockade of inflammation40,68, was upregulated with age in human brain microglia, murine Klotho KO and AD patients’ CSF and nucleus accumbens (Fig. 2i,j). The gene discussed is KL; the disease is Alzheimer disease.