We have generated a collection of 111 genetically barcoded protein kinase null mutants in A. fumigatus and, using Bar-seq mediated competitive fitness profiling reveal a critical role for the dual-specificity tyrosine-regulated kinase (DYRK) YakA in adaptation to iron limitation, temperature, pH, hypoxia and azole drug stress and in contrast to the role played by its orthologue in yeast-type fungi, pathogenicity in a mouse model of infection. Here, WEE1 is linked to infection.