Subsequently, we overexpressed MARCH5 in MHCC-97H using plasmids and knocked down MARCH5 in Huh7 cells by two independent small interfering RNAs (siRNAs) and corresponding results were confirmed by both QRT-PCR and Western blot analyses (Fig. 2c, e).By CCK-8 experiments, we could clearly observe that knockdown of MARCH5 significantly inhibited cell proliferation, while overexpression of MARCH5 significantly promoted tumor cell proliferation (Fig. 2f, g). Here, MARCHF5 is linked to neoplasm.