Therefore, we investigated the effect of MARCH5 on autophagy in hepatocytes, which is consistent with the malignant biological behavior of HCC, and found that stable overexpression of MARCH5 strongly increased the induced transactivation of BECN1 and microtubule-associated protein 1 light chain 3 (LC3)-II, and decreased the p62 level (Fig. 5a). This evidence concerns the gene BECN1 and hepatocellular carcinoma.