Therefore, there is an urgent need to develop novel drugs.<h4>Methods</h4>We investigated the efficacy of a new biological bacteriostatic agent (BBA), composed of lysozyme, phytoalexin, chitosan oligosaccharide, sinensetin, 18β/20α-glycyrrhizin, and betaine, against vaginitis using <i>in vitro</i> and <i>in vivo</i> studies. Here, LYZ is linked to vaginitis.