ASS1 and malignant colon neoplasm: By contrast, in mouse colon cancer cells (MC38) with mutated p53 (ref. 18), we found no elevation in ASS1 nuclear localization following Dox treatment (Extended Data Fig. 2a), whereas in LS-174-T colon cancer cells expressing WT p53 (ref. 19), we again found nuclear expression of ASS1 following treatment with Dox (Extended Data Fig. 2b).