RELA and glioma: We then examined the transfected glioma cells for NF-κB activity; We observed increased p65 (Ser536) phosphorylation in all three cell lines overexpressing NF-κB p65 (Fig. 4A); Furthermore, a NF-κB p65 binding ELISA for relative NF-κB activity on nuclear fraction lysates showed nuclear NF-κB p65 transcriptional activity was significantly increased in all three glioma cells overexpressing NF-κB p65 (Fig. 4B).