TWIST1 and cancer: The scRNA-seq data from four different cancer cell lines (A549, DU145, MCF7, and OVCA420) revealed that signaling pathways known to drive EMT- like TGFβ1, EGF, and TNF- converge on NF-κB and FOSL1, before initiating the expression of typical EMT master regulators such as STAIL, TWIST, and ZEB [75].