To evaluate <i>in vivo</i> antitumor efficacy of manufactured CAR T cells, tumor growth and mouse survival were monitored in a human cervical cancer xenograft model.<h4>Results</h4><i>In vitro</i> experiments demonstrated that knockdown of A2aR alone or in combination with Tim3 significantly improved CAR T cell proliferation, cytokine production, and cytotoxicity in presence of tumor cells in an antigen-specific manner. The gene discussed is ADORA2A; the disease is cervical carcinoma.