TNF and neoplasm: As for the killing-tumor functions of CD8+T cells, we proved that in CT26 mouse model the combined group had highest abilities to secret of TNFα and PFN of CD8+T cells (Fig. 7j-m), while the results of MC38 mouse model showed the combined group expressed highest chemokines of IL2, TNFα, GZMB, and PFN compared with the other groups (Fig. 7n-q).