Then we also disclosed that Rg3 or miR-216a-5p inhibition could obviously (p < 0.001) weakened the upregulation of p-p70s6k and downregulation of p-MARK in the renal tissues of DKD model mice, and the weakening effect of miR-216a-5p inhibition was more obvious (p < 0.001) than that of Rg3 (Figure 4E). This evidence concerns the gene MARK1 and diabetic kidney disease.