Our results suggest that OSCC patients with the variant CC genotype or C allele carriers (CT+CC genotypes) of the TLR9 rs187084 polymorphism have a higher risk of lymph node metastasis and advanced tumor stage, as well as a reduced OS, compared to patients with the TT genotype. This evidence concerns the gene TLR9 and metastatic malignant neoplasm in the lymph nodes.