To address whether MLKL-dependent necroptosis drives skin inflammation also in Casp8E-KO mice, we crossed them to MlklAA/AA mice, which express a mutated version of MLKL that cannot be phosphorylated by RIPK3 due to substitution of serines at positions 345 and 347 to alanines (Supplementary Fig. 1A, B) [26–28]. The gene discussed is MLKL; the disease is dermatitis.