TMT1B and neoplasm: Short hairpin RNA (shRNA)-mediated knockdown of METTL7B (three independent shM7B-a, -b, and -c) was implemented in GIC19 and GIC61 (referred to as GICshM7B) and confirmed at the protein level (Figure S1I), followed by intracerebral injection of GIC19 (scramble [scr] and shM7B-c) into the forebrain of recipient mice, which were then maintained on tumor watch until symptoms arose.