Flow cytometry analysis revealed that selective MIF depletion in macrophages led to a significant reduction in Th1 (CD4+IFNγ+) and Th17 (CD4+IL-17a+) cells in the anti-GBM CGN kidney (Figures 6A, C; Supplementary Figures S2A, C). This evidence concerns the gene IFNG and childhood gender nonconformity.