Findings from the present study added new information that macrophages may mediate anti-GBM CGN via MIF-dependent mechanism as selective depletion of macrophage MIF protected against anti-GBM CGN by inhibiting macrophage infiltration and promoting macrophage polarization from the pro-inflammatory M1 phenotype towards the anti-inflammatory M2 phenotype. This evidence concerns the gene MIF and childhood gender nonconformity.