The study of different fractionation modes combined with immunotherapy in colorectal cancer found that the results of radiotherapy combined with immunosuppressants at three biologic effect doses of 16.4Gy×1, 8Gy×3 and 2Gy×18 showed that 8Gy×3 was the best segmentation mode, which significantly increased the number of tumor-infiltrating lymphocytes and the expression of PD-L1 and TIGIT, and could most effectively control tumor development (90% complete response) after combining ICI (46). This evidence concerns the gene CD274 and colorectal cancer.