Additionally, this study targets at preliminarily clarifying the biological roles of NSUN2 and HGH1 in BC.<h4>Methods</h4>Tumor and adjacent tissues from 5 BC patients were collected, and the m<sup>5</sup>C modification target HGH1 in BC was screened through RNA sequencing (RNA-seq) and single-base resolution m<sup>5</sup>C methylation sequencing (RNA-BisSeq). The gene discussed is NSUN2; the disease is breast cancer.