CAMK2A and Anxiety: To check whether endogenous glutamatergic neurons in the MEPV (MePVGlu neurons) are involved in the regulation of the natural sleep-wake cycle or anxiety-like behaviors, we injected AAV-CaMKIIa-GCaMP6f into the MePV of C57BL/6J mice (MePVGlu-GCaMP6f mice) and recorded changes in Ca2+ signal during sleep state transitions using the electroencephalogram and electromyogram (EEG-EMG) system or anxiety state transitions during behavioral tests (Figure 1A and Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.176329DS1).