Interestingly, G1/S expression levels were significantly higher in tumors resected from the DYRK1A KO animals compared to NT control tumors, highly consistent with what observed in vitro with the DYRK1A KO and harmine treated colon cancer cells (Fig. 5e) When assessing expression levels of the G2/M regulators, no significant changes were observed in the cyclin B1, PLK1 and aurora B levels, possibly due to the low numbers of animal used in these preclinical experiments. This evidence concerns the gene CCNB1 and malignant colon neoplasm.