Although ZIKV infection downregulates TLR7 and TLR8 mRNA expression in human monocytes [45], we did not observe any effect of VitD3 treatment on TLR7 and TLR8 mRNA expression in either ZIKV-Mon or ZIKV-VitD3-Mon when compared to VitD3-Mon. The gene discussed is TLR8; the disease is Zika virus infectious disease.