To decipher the functional relevance of the observed PSC-induced microbiota alterations on colitis severity, we next reconstituted germ-free wild-type mice with stool derived from mice with sclerosing cholangitis (Mdr2−/− mice) or without sclerosing cholangitis (wild-type mice), respectively, and induced colitis in these mice using a blocking anti-IL10Rα mAb32 (figure 4A). This evidence concerns the gene IL10RA and sclerosing cholangitis.