CRISPR‐Cas9 was initially employed to knock out TFG expression in HEK293T, N2a, and NSC‐34 cells for two primary reasons: First, TFG c.177A>C (p.(Lys59Asn)) causes autosomal recessive HSP in its homozygous status, and second, TFG protein is ubiquitously expressed in neuronal and non‐neuronal cells. This evidence concerns the gene TFG and hereditary spastic paraplegia.