A similar hepatic steatosis with CD9 ablated mice was observed, manifesting as increased BW, LW, LW/BW, liver TG content, lipid accumulation and fatty acid metabolic genes and proteins changed under HFD condition, although NCD‐fed CFD‐overexpressed mice appeared unchanged compared with controls (Figure 3I‐L; Figures S5C‐E and S6, Supporting Information). This evidence concerns the gene CD9 and fatty liver disease.