SP1 and infection: After 72hours, the cells were either mock-infected or infected with AAV2 at amultiplicity of infection (MOI) of 20,000 and 24 h later subjected to chromatinimmunoprecipitation (ChIP) assays using an anti-Sp1 antibody and primers for thep5 (Fig. 14A) or p19 (Fig. 14B) promoter regions, respectively.