FOS and migraine disorder: When repeatedly stimulated at the GV16 acupoint, mice with NTG-induced peripheral hypersensitivity and high TNC c-Fos expression can reduce hindlimb and face mechanical and cold allodynia. DBV injections at GV16 activate alpha-2 adrenoceptors, not endogenous opioid receptors. Based on this study, chemotherapeutic acupuncture with Diluted Bee Venom at GV16 may help migraine patients with peripheral discomfort.