To evaluate whether oAd/APP complex can effectively infect our target B16-F10 murine melanoma cells to induce expression of therapeutic genes, B16-F10 cells were infected with naked oAd or oAd/APP at an MOI of 10, 20, or 50 and the expression level of IL-12 or GM-CSF was measured by ELISA. This evidence concerns the gene CSF2 and melanoma.