However, due to the limited number of AFP-negative patients in this expanded cohort, we found that serum IL-41 expression was higher than the diagnostic cutoff (46.87) in five newly added patients but lower in 2 patients with hepatic hemangioma (not shown), resulting in a diagnostic accuracy of 100% among these newly added liver cancer patients (Figure 5B). This evidence concerns the gene AFP and Hepatic hemangioma.