IQGAP1 and acute myeloid leukemia: Before proceeding further, western blot of the five fractions: index whole cell lysate of the AML and the fractions that did not bind to and ones that were eluted off the affinity columns sequentially were probed with a murine anti-IQGAP1 antibody (Santa Cruz Biotechnology Inc, catalog number: SC-374307) to confirm that IQGAP1 was indeed present in the immunoaffinity purified fraction.