The results displayed that cleaved IL-1β, cleaved Caspase 1, GSDMD-N-terminal, and NLRP3 were promoted in the AAV9-control-infected heart of MI mice, and a significantly higher expression of them in the AAV9-miR-654-3p sponge-transfected MI mice (Fig. 5K, L). The gene discussed is GSDMD; the disease is myocardial infarction.