Besides, we detected the myocardial enzyme Cardiac troponin I (cTNI) and heart failure marker NT-proBNP to verify the AAV9-miR-654-3p sponge-induced injury post-MI, which exhibited a significant promotion in the AAV9-miR-654-3p sponge group compared with the AAV9-control group (Fig. 5I). Here, NPPB is linked to heart failure.