Meanwhile, the mRNA levels of ANP, BNP, COL I, and COL III also indicated that Sema significantly attenuated TAC-induced myocardial hypertrophy and fibrosis (Fig. 2K). Parallel transcriptomic analysis of myocardial tissue from mice in the reversal experiment revealed that the treatment of Sema significantly rescued TAC-induced changes at the gene level (Fig. S2A). The gene discussed is NPPB; the disease is cardiac hypertrophy.