To corroborate whether IMMT ubiquitination, mediated by the KLHL9/KLHL13/CUL3 complex, impacts the virulence and intracellular viability of B. pseudomallei in vivo, BALB/c mice were subjected to infection with B. pseudomallei WT or ΔbipD via intraperitoneal injection, along with MLN4924 treatment. Here, CUL3 is linked to infection.