CD8A and non-small cell lung carcinoma: To further investigate the correlation between ACLS and PFS in patients with advanced NSCLC, firstly, X-tile software was used to obtain the optimal cutoff values of AC of CD3+ (898 cells/μL), CD4+ (492 cells/μL), CD8+ (292 cells/μL), B (99 cells/μL) and NK cells (91 cells/μL); then the patients were divided into high lymphocyte subsets and low lymphocyte subsets groups according to the cutoff value, and the differences in PFS between the two groups were compared.