In the present study, we were surprised to find that nest granulosa cell‐specific knock‐in of miR‐3061+/−/AMH‐Cre+/− in microRNA‐3061 transgenic mice resulted in the downregulated expression of the transcription factor PAX7 and Wnt/Ca2+ signalling pathway genes; this phenomenon was also manifested in the follicular fluid exosomes of POF patients. The gene discussed is AMH; the disease is premature menopause.