To further explore whether the in vitro results of AMPK activation by metformin were consistent with the in vivo results, we examined the expression patterns of AMPK, ACC, mTOR, p70S6K, p-AMPK, p-ACC, p-mTOR, p-p70S6K, PCAF, and H3K9ac in tumor tissues collected from the cervical cancer xenograft nude mouse model. The gene discussed is PRKAA1; the disease is neoplasm.