Finally, we delved into the EGFR downstream signaling pathway and observed that several EGFR signaling-related proteins such as p-AKT, p-STAT3, JNK, and p-ERK were decreased upon GLUT1 knockdown (Fig. 4J, K, and S4I, Supplementary information), suggesting GLUT1 could activate EGFR signaling pathway in lung cancer. Here, STAT3 is linked to lung cancer.