After seven days of OI, we found that the expression levels of RUNX2 (0.61-fold, P < 0.05) and Osterix (0.35-fold, P < 0.01) were significantly lower than those in the shRNA-YAP-NC group, and OCN expression in the shRNA-YAP groups tended to be lower than that in the Blank group and the shRNA-YAP-NC group, however, the differences were not statistically significant. This evidence concerns the gene YAP1 and osteogenesis imperfecta.