We compared images from subsequent staining cycles from an AD Braak IV/V hippocampus stained with anti-Neu antibody (secondary fluorophore 568) and anti-FICD antibody (secondary fluorophore 488) in round 1 and anti-Tau (multiple isoforms) antibody (secondary antibody 488) and anti-HSPA8 antibody (secondary fluorophore 568) used in cycle 2. The gene discussed is HSPA8; the disease is Alzheimer disease.