These results of this study suggest that one FDEGs (TNFAIP3) and five ADEGs (CDKN1A, HSPA5, MAPK8IP1, PPP1R15A, and VEGFA) may be noteworthy differentially expressed genes associated with keratoconus, and they have potential diagnostic efficiency in the disease. This evidence concerns the gene HSPA5 and keratoconus.