To examine the effect of two LCA-associated CCT2 missense mutations in the mammalian retina, we generated two knock-in mouse strains carrying Cct2 NM_007636.3:c.1411A > C p.(T400P) (hereafter, T400P) and NM_007636.3:c.1760_1761delinsAC p.(R516H) (hereafter, R516H) mutations, respectively (Fig. 1a). The gene discussed is CCT2; the disease is Leber congenital amaurosis.