Furthermore, in agreement with previous studies indicating that the knockout of SHMT2 increases ROS production in human cancer cells [63], dTMP supplementation reduced DHE staining of about 50% (Fig. 6E, F) in eye discs from RasV12 4DP-fed larvae, thus suggesting that under PLP deficiency dTMP depletion, may also contribute to increase the oxidative stress, besides compromising repair (Fig. 6G). This evidence concerns the gene SHMT2 and cancer.